As a conclusion to the participatory approach undertaken, the stakeholders expressed a better understanding of the challenges of reconciling urban development and preservation of connectivity, and the importance of collaboration in responding to them. They also expressed their willingness to defend the transformative scenario, which means not respecting certain planned urban developments. This could be reflected in public policies for the coming years, questioning in another way the trade-offs between urban development and the preservation of biodiversity. Therefore, we argue that this approach permitted to fill a gap in the use of ecological network approaches in the spheres of science and policy interdependencies, with the aim of a stronger consideration of biodiversity conservation issues in metropolitan governance.